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EC Number	Application	Commentary	Reference
1.14.13.172	synthesis	biosynthetic pathway for maleate production in engineered Escherichia coli. Expression of salicylate 5-hydroxylase, introduction of salicylate biosynthetic pathway and gentisate ring cleavage pathway allow the synthesis of maleate from glycerol. Further optimizations boost maleate titer to 2.4 g/l in shake flask experiments. Subsequent scale-up biosynthesis of maleate in a 3-l bioreactor under fed-batch culture conditions enables the production of 14.5 g/l of maleate	764345

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Release 2023.1 (January 2023)