EC Number |
Application |
Reference |
---|
1.13.12.6 | analysis |
Cypridina noctiluca luciferase is utilized for biochemical and molecular biological applications, including bioluminescent enzyme immunoassays, far-red luminescence imaging, and high-throughput reporter assays |
746376 |
1.13.12.6 | analysis |
dual reporter assay using one luciferase for monitoring gene expression and a second as an internal control, based on secreted luciferases from Cypridina noctiluca and Gaussia princeps that do not require lysis or special equipment. The assay can be carried out sequentially, the activities are high and can be measured in a small volume and a simple procedure. Development of a one-tube reporter assay for the two enzymes |
685701 |
1.13.12.6 | analysis |
intramolecular bioluminescence resonance energy transfer system consisting of a fusion protein of the enzyme and enhanced yellow fluorescent protein for investigating peptide processing in living cells |
654184 |
1.13.12.6 | analysis |
the secreted Cypridina luciferase (CLuc) is used as an ex vivo indicator to continuously monitor tumor progression. On the other hand, the non-secreted firefly luciferase is used as an in vivo indicator to analyze the spatial distribution of the tumor at suitable time points indicated by CLuc. Tumor monitoring systems using dual luciferases are available, allowing long-term bioluminescence imaging under minimal stress for the experimental animals, e.g. BALB/cAJcl-nu/nu mice. Continuous non-invasive measurement of CLuc activity for tumor growth allows us to determine a suitable time to assess tumor spatial distribution and growth, thereby, reducing the animal stress and the experimental cost |
744013 |
1.13.12.6 | analysis |
the thermostable enzyme can be used for various research applications, including in vivo imaging and high throughput reporter assays |
764716 |
1.13.12.6 | analysis |
use of enzyme as a reporter in a screening system of Saccharomyces cerevisiae to improve secretion efficiency in yeast |
684849 |
1.13.12.6 | biotechnology |
use of enzyme as a potent secreted reporter |
655065 |
1.13.12.6 | diagnostics |
Cypridina luciferase is used as bioluminescence reporter enzyme in yeast, bacterial, and mammalian cell-based assays, enzyme activity is measured with a luminometer by addition of native or synthetic luciferin, also known as Vargulin, 0.5 microM in 10 mM Tris-HCl, pH 7.4 |
700006 |
1.13.12.6 | diagnostics |
far-red luminescence imaging technology to visualize tumor specific antigens on cell surfaces in vitro (human hepato-cellular carcinoma cell line Huh-7) and in living bodies (mice xenografted with human Delta-like protein-positive Huh-7 cells), based on a far-red fluorescent indocyanine derivative conjugated to biotinylated Cypridina luciferase linked to an anti-human Delta-like protein (Dlk-1) monoclonal antibody (embryonic antigens expressed on the surface of many cancer cells) via biotin-avidin interaction |
700998 |
1.13.12.6 | diagnostics |
labeling of prostaglandin E2 with the bioluminescent enzyme Cypridina luciferase linked to monoclonal anti-prostaglandin E2 antibody as enzyme-linked immunosorbent assay (ELISA) system, detection of 7.8-500 pg/ml prostaglandin E2 |
699954 |