EC Number |
Application |
Reference |
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1.13.12.2 | biotechnology |
immobilization of L-lysine-2-monooxygenase on an electrode surface, via polymerization of polyvinyl alcohol, provides a biosensor that detects L-lysine concentrations down to 0.01 mM |
440262 |
1.13.12.2 | biotechnology |
immobilization on silica gel provides a flow-through analyzer for concentrations between 5.5 and 55 mM L-lysine at pH 8.2, it retains 50% activity after two months |
440263 |
1.13.12.2 | synthesis |
2-monooxygenase (DavB) and delta-aminovaleramidase (DavA) are co-expressed in Escherichia coli BL21(DE3) to produce nylon-5 monomer 5-aminovalerate from L-lysine. PP2911 (4-aminobutyrate transporter in Pseudomonas putida) and LysP (the lysine specific permease in Escherichia coli) are overexpressed to promote 5-aminovalerate production using whole cells of recombinant Escherichia coli. The constructed Escherichia coli strain overexpressing transport proteins exhibits good 5-aminovalerate production performance and might serve as a promising biocatalyst for 5-aminovalerate production from L-lysine. This strategy not only shows an efficient process for the production of nylon monomers but also might be used in production of other chemicals |
746458 |
1.13.12.2 | synthesis |
installation of an Escherichia coli whole-cell biocatalytic system, environmentally friendly, for industrial production of 5-aminovalerate using recombinant enzymes 5-aminovaleramide amidohydrolase and L-lysine 2-monooxygenase, overview |
745567 |
1.13.12.2 | synthesis |
L-lysine is a potential feedstock for the production of bio-based precursors for engineering plastics. Usage of the enzyme in a microbial process for high-level conversion of L-lysine into 5-aminovalerate that can be used as a monomer in nylon 6,5 synthesis, method overview |
744567 |
1.13.12.2 | synthesis |
the enzyme can be used for production of 5-aminovalerate, a potential C5 platform chemical for synthesis of valerolactam, 5-hydroxyvalerate, glutarate, and 1,5-pentanediol. Escherichia coli is engineered for production of 5-aminovalerate from L-lysine by coupled reaction of recombinant DavB, L-lysine monooxygenase, and recombinant DavA, 5-aminovaleramidase. Because L-lysine is an industrial fermentation product, the two-enzyme coupled system presents a promising alternative for the production of 5-aminovalerate |
746434 |