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development of a highly sensitive one-step PLA-enzyme immunoassay and an immunostrip, usable as a rapid test under field conditions. The PLA-immunometric tests allow a rapid and easy detection of Yersinia pestis in environmental and flea samples
multiplex real-time polymerase chain reaction assay for the detection of Yersinia pestis and Yersinia pseudotuberculosis using the Yersinia pestis pla gene coding for plasminogen activator and the ypo2088 gene. Assays are both sensitive and specific, the lower detection limit is 10-100 fg of extracted total DNA
the Lpp Pla double mutant of Yersinia pestis CO92 is highly attenuated and it retains the ability to elicit innate and subsequent acquired immune responses in the host similar to that of wild-type CO92, which are highly desirable in a live-attenuated vaccine candidate
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