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synthesis
simplified method to prepare Mg-protoporphyrin monomethyl ester from freeze dried BchE mutant Rhodobacter capsulatus DB575 cells by extraction with acetone/H2O/25% NH3. Isolated Mg-protoporphyrin monomethyl ester can be identified by absorption and fluorescence spectroscopy, and its purity analyzed by HPLC. The extracted Mg-protoporphyrin monomethyl ester can be dried and redissolved in buffered DMSO to be used as substrate. Mg-protoporphyrin monomethyl ester is in addition a substrate to magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase, EC 1.13.14.81
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