EC Number   |
Application  |
Reference |
|---|
   1.14.14.3 | agriculture |
engineering of broad-host-range Erwinia amylovora virus Y2 to enhance its killing activity and for use as a luciferase reporter phage. The reporter phage Y2::luxAB transduces bacterial luciferase into host cells and induces synthesis of large amounts of a LuxAB luciferase fusion. After the addition of aldehyde substrate, bioluminescence can be monitored, and enables rapid and specific detection of low numbers of viable bacteria |
744110 |
| 1.14.14.3 | agriculture |
optimization of fused luxAB expression, quantum yield and application as a reporter gene in plant protoplasts. Luciferase activity is dramatically increased upon use of the optimized gene and the 35S promoter compared to the original luxAB in Arabidopsis and maize cells |
746250 |
| 1.14.14.3 | analysis |
a minimized cascade for Lux with greater ease of use, utilizes a chemoenzymatic reaction with biomimetic nicotinamide 1-benzyl-1,4-dihydronicotinamide in place of the flavin reductase reaction in the Lux system. The minimized cascade reaction can be applied to monitor bioluminescenceof the Lux reporter in eukaryotic cells effectively, and can achieve higher efficiencies than the system with flavin reductase |
765342 |
| 1.14.14.3 | analysis |
bioluminescent assay based on a system of coupled enzymatic reactions catalyzed by bacterial luciferase and NADH:FMN-oxidoreductase to monitor toxicity and antioxidant activity of bioactive compounds such as fullerenols, perspective pharmaceutical agents, nanosized particles, water-soluble polyhydroxylated fullerene-60 derivatives. Fullerenols suppress bioluminescent intensity at concentrations above 0.01 g/l and above 0.001 g/l for C60O2-4(OH)20-24 and Fe0.5C60(OH)xOy, respectively |
745976 |
| 1.14.14.3 | analysis |
enzyme can be used to monitor changes in gene expression as a reporter system in slow-growing mycobacteria, i.e. Mycobacterium tuberculosis strain H37Ra, determination of recombinant enzyme decay rate |
658778 |
| 1.14.14.3 | analysis |
expression of the bacterial luciferase system in mammalian cells for generation of bioreporters for in vivo monitoring and diagnostics technology, method evaluation and optimization, overview |
675217 |
| 1.14.14.3 | analysis |
fusion of circularly permuted Venus, a bright variant of yellow fluorescent protein, to the C-terminus of subunit LuxB to induce bioluminescence resonance energy transfer (BRET). By using decanal as added substrate, color change and ten-times enhancement of brightness is achieved in Escherichia coli upon expression. Expression of the Venus-fused luciferase in human embryonic kidney cell lines or in Nicotiana benthamiana leaves together with the substrate biosynthesis-related genes (luxC, luxD and luxE) enhances the autonomous bioluminescence |
765786 |
| 1.14.14.3 | analysis |
high-throughput, homogeneous, bioluminescent assay for Pseudomonas aeruginosa gyrase inhibitors and other DNA-damaging agents based on a Photorhabdus luminescens luciferase operon transcriptional fusion to a promoter that responds to DNA damage caused by reduced gyrase levels and fluoroquinoline inhibition |
680943 |
| 1.14.14.3 | analysis |
sensitive and selective bacterial luminescence method for the detection of pyruvic acid based on lactate dehydrogenase and the bacterial luciferase-FMN:NADH oxidoreductase bioluminescence in vitro. NADH involved in the LDH reaction system can be quantitatively analyzed by the bioluminescence system. A good linear relationship between the luminescence intensity and pyruvic acid concentration is observed within the range of 0.000140.001 mol/l, and the pyruvic acid detection limit is 0.000085 mol/l |
745979 |
| 1.14.14.3 | analysis |
the enzyme is used as a reporter system tool for analysis of promoter and gene expression activity, overview |
-, 711341 |
