EC Number   |
Application |
Reference |
|---|
   1.14.14.3 | analysis |
a minimized cascade for Lux with greater ease of use, utilizes a chemoenzymatic reaction with biomimetic nicotinamide 1-benzyl-1,4-dihydronicotinamide in place of the flavin reductase reaction in the Lux system. The minimized cascade reaction can be applied to monitor bioluminescenceof the Lux reporter in eukaryotic cells effectively, and can achieve higher efficiencies than the system with flavin reductase |
765342 |
| 1.14.14.3 | analysis |
bioluminescent assay based on a system of coupled enzymatic reactions catalyzed by bacterial luciferase and NADH:FMN-oxidoreductase to monitor toxicity and antioxidant activity of bioactive compounds such as fullerenols, perspective pharmaceutical agents, nanosized particles, water-soluble polyhydroxylated fullerene-60 derivatives. Fullerenols suppress bioluminescent intensity at concentrations above 0.01 g/l and above 0.001 g/l for C60O2-4(OH)20-24 and Fe0.5C60(OH)xOy, respectively |
745976 |
| 1.14.14.3 | agriculture |
engineering of broad-host-range Erwinia amylovora virus Y2 to enhance its killing activity and for use as a luciferase reporter phage. The reporter phage Y2::luxAB transduces bacterial luciferase into host cells and induces synthesis of large amounts of a LuxAB luciferase fusion. After the addition of aldehyde substrate, bioluminescence can be monitored, and enables rapid and specific detection of low numbers of viable bacteria |
744110 |
| 1.14.14.3 | analysis |
enzyme can be used to monitor changes in gene expression as a reporter system in slow-growing mycobacteria, i.e. Mycobacterium tuberculosis strain H37Ra, determination of recombinant enzyme decay rate |
658778 |
| 1.14.14.3 | molecular biology |
enzyme can be used to monitor changes in gene expression as a reporter system in slow-growing mycobacteria, i.e. Mycobacterium tuberculosis strain H37Ra, determination of recombinant enzyme decay rate |
658778 |
| 1.14.14.3 | biotechnology |
establishment and evaluation of the enzyme used in a luciferase-based reporter system, pPL2lux, harboring the listerial secA and hlyA promoters translationally fused to luxABCDE, overview |
671457 |
| 1.14.14.3 | medicine |
expression in Staphylococcus aureus Xen29. In the absence of antibiotics, staphylococcal bioluminescence increases over time until a maximum after ca. 6 h of growth, and subsequently decreases to the detection threshold after 24 h of growth. Up to minimal inhibitory concentrations of the antibiotics vancomycin, ciprofloxacin, erythromycin or chloramphenicol, bioluminescence increases according to a similar pattern up to 6 h of growth, but after 24 h, bioluminescence is higher than in the absence of antibiotics. Antibiotic pressure impacts the relation between bioluminescence per organism and bioluminescence. Under antibiotic pressure, bioluminescence is not controlled by luxA expression but by cofactors impacting the bacterial metabolic activity |
745081 |
| 1.14.14.3 | analysis |
expression of the bacterial luciferase system in mammalian cells for generation of bioreporters for in vivo monitoring and diagnostics technology, method evaluation and optimization, overview |
675217 |
| 1.14.14.3 | diagnostics |
expression of the bacterial luciferase system in mammalian cells for generation of bioreporters for in vivo monitoring and diagnostics technology, method evaluation and optimization, overview |
675217 |
| 1.14.14.3 | analysis |
fusion of circularly permuted Venus, a bright variant of yellow fluorescent protein, to the C-terminus of subunit LuxB to induce bioluminescence resonance energy transfer (BRET). By using decanal as added substrate, color change and ten-times enhancement of brightness is achieved in Escherichia coli upon expression. Expression of the Venus-fused luciferase in human embryonic kidney cell lines or in Nicotiana benthamiana leaves together with the substrate biosynthesis-related genes (luxC, luxD and luxE) enhances the autonomous bioluminescence |
765786 |
