EC Number |
Organic Solvent |
Reference |
---|
3.4.19.3 | acetonitrile |
at 50% (v/v) concentrations more than 50% of the initial activity is retained |
-, 727453 |
3.4.19.3 | Ethanol |
the enzyme retains stability at concentrations up to 90% (v/v) |
-, 727453 |
3.4.19.3 | guanidine-HCl |
approximately 70% of the original activity is retained after preincubation with 10 mM DTT, 50 mM Na-phosphate buffer (pH 7.0) containing less than either 0.01% SDS, 1 M urea, or 1 M guanidine-HCl at 37°C for 15 min |
722561 |
3.4.19.3 | isopropanol |
at 50% (v/v) concentrations more than 50% of the initial activity is retained |
-, 727453 |
3.4.19.3 | Methanol |
the enzyme retains stability at concentrations up to 75% (v/v) |
-, 727453 |
3.4.19.3 | SDS |
approximately 70% of the original activity is retained after preincubation with 10 mM DTT, 50 mM Na-phosphate buffer (pH 7.0) containing less than either 0.01% SDS, 1 M urea, or 1 M guanidine-HCl at 37°C for 15 min |
722561 |
3.4.19.3 | urea |
approximately 70% of the original activity is retained after preincubation with 10 mM DTT, 50 mM Na-phosphate buffer (pH 7.0) containing less than either 0.01% SDS, 1 M urea, or 1 M guanidine-HCl at 37°C for 15 min |
722561 |