acetonitrile
at 50% (v/v) concentrations more than 50% of the initial activity is retained
Ethanol
the enzyme retains stability at concentrations up to 90% (v/v)
guanidine-HCl
approximately 70% of the original activity is retained after preincubation with 10 mM DTT, 50 mM Na-phosphate buffer (pH 7.0) containing less than either 0.01% SDS, 1 M urea, or 1 M guanidine-HCl at 37°C for 15 min
isopropanol
at 50% (v/v) concentrations more than 50% of the initial activity is retained
Methanol
the enzyme retains stability at concentrations up to 75% (v/v)
SDS
approximately 70% of the original activity is retained after preincubation with 10 mM DTT, 50 mM Na-phosphate buffer (pH 7.0) containing less than either 0.01% SDS, 1 M urea, or 1 M guanidine-HCl at 37°C for 15 min
urea
approximately 70% of the original activity is retained after preincubation with 10 mM DTT, 50 mM Na-phosphate buffer (pH 7.0) containing less than either 0.01% SDS, 1 M urea, or 1 M guanidine-HCl at 37°C for 15 min
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