EC Number   |
Natural Substrates |
|---|
    6.3.4.5 | ATP + L-citrulline + L-Asp |
argininosuccinate synthase catalyzes the rate-limiting step in the recycling of citrulline to arginine, which in endothelial cells, is tighly coupled to the production of nitric oxide. The upstream open reading frame found in the extended, overlapping 5'-untranslated region of argininosuccinate synthase mRNA species suppresses endothelial argininosuccinate expression providing a novel mechanism for regulating endothelial NO production by limiting the availability of arginine |
| 6.3.4.5 | ATP + L-citrulline + L-Asp |
argininosuccinate synthase expression is required to maintain nitric oxide production and cell viability in aortic endothelial cells |
| 6.3.4.5 | ATP + L-citrulline + L-Asp |
IL-1beta stimulates argininosuccinate synthetase gene expression through NF-kappaB in Caco-2 cells |
| 6.3.4.5 | ATP + L-citrulline + L-Asp |
the substrate for NO synthesis, L-arginine can be regenerated from the L-citrulline the coproduct of nitric-oxide synthase. This requires the sequential action of two enzymes, argininosuccinate synthetase and argininosuccinate lyase |
| 6.3.4.5 | ATP + L-citrulline + L-Asp |
mechanism to recycle citrulline, formed in the nervous system via nitric oxide synthetase, EC 1.14.13.39, activity, back to the nitric oxide precursor, L-Arg |
| 6.3.4.5 | ATP + L-citrulline + L-Asp |
third reaction of urea cycle |
| 6.3.4.5 | ATP + L-citrulline + L-Asp |
anabolic function in biosynthesis of Arg and catabolic function as the first enzyme of citrulline utilization as nitrogen source |
| 6.3.4.5 | ATP + L-citrulline + L-Asp |
urea-cycle enzyme |
| 6.3.4.5 | ATP + L-citrulline + L-aspartate |
mediates transformation of L-citrulline to L-arginine in the ureum cycle in hepatocytes, first step in L-citrulline recycling pathway |
| 6.3.4.5 | ATP + L-citrulline + L-aspartate |
penultimate step in the biosnthesis of arginine, rate-limiting enzyme of both the urea and arginine-citrulline cycles |
