EC Number |
Natural Substrates |
---|
6.1.1.23 | ATP + Asp + tRNAAsn |
while large amounts of Asp-tRNAAsn are detrimental to Escherichia coli with trypA34 missense mutation, a smaller amount supports protein synthesis and allows the formation of up to 38% of the wild-type level of missense-suppressed tryptophan synthetase |
6.1.1.23 | ATP + Asp + tRNAAsn |
while large amounts pf Asp-tRNAAsn are detrimental to Escherichia coli with trypA34 missense mutation, a smaller amount supports protein synthesis and allows the formation of up to 38% of the wild-type level of missense-suppressed tryptophan synthetase |
6.1.1.23 | ATP + L-aspartate + tRNAAsn |
- |
6.1.1.23 | ATP + L-aspartate + tRNAAsn |
the product L-aspartyl-tRNAAsn is transamidated by amidotransferase to form Asn-tRNAAsn. Synthesis of Asn-tRNA via the indirect pathway |
6.1.1.23 | ATP + L-aspartate + tRNAAsn |
Thermus thermophilus deprived of asparagine synthetase snthesizes Asn on tRNAAsn via a tRNA-dependent pathway involving a nondiscriminating aspartyl-tRNA synthetase that charges Asp onto tRNAAsn prior to conversion of the Asp to Asn by GatCAB |
6.1.1.23 | ATP + L-aspartate + tRNAAsn |
dual activity of the ND-AspRS since an asparaginyl-tRNA synthetase is missing in Sulfolobus tokodaii strain 7 |
6.1.1.23 | ATP + L-aspartate + tRNAAsn |
reaction via transamidation mechanism |
6.1.1.23 | ATP + L-aspartate + tRNAAsp |
- |
6.1.1.23 | ATP + L-aspartate + tRNAAsx |
- |
6.1.1.23 | more |
in bacteria that lack AsnRS, AspRS is nondiscriminating and generates both Asp-tRNAAsp and the noncanonical, misacylated Asp-tRNAAsn, this misacylated tRNA is subsequently repaired by the glutamine-dependent Asp-tRNAAsn/Glu-tRNAGln amidotransferase, EC 6.3.5.6, increasing tRNAAsp specificity in an ND-AspRS diminishes in vivo toxicity |