EC Number   |
Natural Substrates |
|---|
    6.1.1.12 | ATP + L-aspartate + tRNAAsn |
- |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsn |
the archaeal AspRS enzyme is nondiscriminating, which means that it forms Asp-tRNAAsp and Asp-tRNAAsn, which is the intermediate in AsntRNAAsn generation by ASp-tRNAAsn amidotransferase, in contrary bacterial enzymes are discriminating ones |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsn |
the archaeal AspRS2 enzyme is discriminating, which means that it forms only Asp-tRNAAsp and not Asp-tRNAAsn, the L1 loop exchange mutant is rendered non-dicriminating |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsn |
the archaeal AspRS2 enzyme is nondiscriminating, which means that it forms Asp-tRNAAsp and Asp-tRNAAsn |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsn |
non-discriminating AspRS2 |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsp |
- |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsp |
protein-RNA recognition between the enzyme and tRNA is highly specific and essential for cell viability |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsp |
specific amino acid binding by the enzyme is required for correct translation of the genetic code |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsp |
the yeast AspRS not only binds and aminoacylates tRNAAsp but also binds its yeast mRNA and initiates retro-inhibition of its expression, overview |
| 6.1.1.12 | ATP + L-aspartate + tRNAAsp |
enzyme deficiency or mutation is involved in development of autosomal recessive disease leukoencephalopathy with brain stem and spinal cord involvement and lactate elevation, i.e. LBSL, often manifesting in early childhood, overview |
