EC Number   |
Natural Substrates |
|---|
   4.6.1.24 | more |
barnase catalyses the overall hydrolysis of single-stranded RNA preferentially at guanylyl residues, yielding new guanosine 3'-phosphate and 5'-OH ends, in a two-step process with cleavage of the RNA chain by transesterification of a 5'-phosphoester bond to form a guanosine 2',3'-cyclic phosphate terminus in the first step, followed by its hydrolysis to a 3'-phosphate product in the second independent step |
| 4.6.1.24 | more |
binase catalyses the overall hydrolysis of single-stranded RNA preferentially at guanylyl residues, yielding new guanosine 3'-phosphate and 5'-OH ends, in a two-step process with cleavage of the RNA chain by transesterification of a 5'-phosphoester bond to form a guanosine 2',3'-cyclic phosphate terminus in the first step, followed by its hydrolysis to a 3'-phosphate product in the second independent step |
| 4.6.1.24 | RNA + H2O |
two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp with 2,3'-cyclic phosphate intermediates |
