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EC Number
Natural Substrates
Commentary (Nat. Sub.)
3.6.1.39
dTTP + H2O
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3.6.1.39
dTTP + H2O
mechanisms of DNA-dependent and-independent dTTP hydrolysis by the gene 4 protein of bacteriophage T7 differ in the pathways by which these reactions are catalyzed. In the presence of dTTP, gene 4 protein monomers assemble as a ring that binds single-stranded DNA and couples the hydrolysis of dTTP to unidirectional translocation and the unwinding of duplex DNA, overview
3.6.1.39
dTTP + H2O
dTTP hydrolysis is coupled to DNA unwinding, dependence of dTTP hydrolysis rates on dsDNA stability, the force-producing step during DNA unwinding is not associated with dTTP binding, but dTTP hydrolysis or phosphate release
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