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EC Number
Natural Substrates
Commentary (Nat. Sub.)
3.2.1.68
amylopectin + H2O
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3.2.1.68
Arabidopsis starch + H2O
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3.2.1.68
beta-limit dextrin + H2O
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3.2.1.68
glycogen + H2O
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3.2.1.68
maize starch + H2O
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3.2.1.68
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involved in starch synthesis
3.2.1.68
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involved in synthesis of reserve and leaf starches
3.2.1.68
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AtISA1 and AtISA2 are required for the production of a functional isoamylase-type of debranching enzyme named Iso1, the major isoamylase found in leaves. The absence of Iso1 leads to an 80% decrease in the starch content and to accumulation of water-soluble polysaccharides whose structure is similar to glycogen
3.2.1.68
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AtISA1/AtISA2 isoamylase influences glucan branching pattern
3.2.1.68
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glucan debranching occurs primarily at the granule surface via ISA3, but in its absence soluble branched glucans are debranched in the stroma via limit dextrinase. Isoamylase acts at the surface of the starch granule. Atisa3 mutants have more leaf starch and a slower rate of starch breakdown than wild-type plants
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