EC Number   |
Natural Substrates |
|---|
   3.2.1.132 | chitosan + H2O |
- |
| 3.2.1.132 | chitosan + H2O |
best substrate |
| 3.2.1.132 | chitosan + H2O |
Chitosan, or beta-1,4-linked glucosamine, is a deacetylated derivative of chitin. The chitosanase is specific for chitosan as substrate |
| 3.2.1.132 | chitosan + H2O |
chitosanase is a glycosyl hydrolase that endolytically hydrolyzes beta-1,4-linkages between D -glucosamine residues in a partially acetylated chitosan |
| 3.2.1.132 | chitosan + H2O |
100% activity with 50% deacetylated chitosan, 89% activity with 70% deacetylated chitosan, 91% activity with 98% deacetylated chitosan |
| 3.2.1.132 | chitosan + H2O |
100% activity with chitosan of 60% degrees of deacetylation |
| 3.2.1.132 | chitosan + H2O |
the enzyme hydrolyzes 70% deacetylated chitosan faster than fully deacetylated chitosan |
| 3.2.1.132 | chitosan + H2O |
the wild type chitosanase shows a clear preference for chitosan with high degrees of N-deacetylation (84-97%) |
| 3.2.1.132 | chitosan + H2O |
chitosan polymers with various degrees of deacetylation (10-94%) are susceptible to hydrolysis by both isoforms. Polymers with 50-70% deacetylation are most susceptible to hydrolysis by isoform A and polymers with 40-80% deacetylation are most susceptible to isoform B |
| 3.2.1.132 | chitosan pentasaccharide + H2O |
- |
