EC Number |
Natural Substrates |
---|
3.1.30.1 | DNA + H2O |
- |
3.1.30.1 | DNA + H2O |
functional role in recombination and DNA repair in vivo |
3.1.30.1 | DNA + H2O |
75000 times more active on single-stranded than on native DNA |
3.1.30.1 | DNA + H2O |
native DNA hydrolysis probably by contaminating nuclease, its inactivation possible at 55°C |
3.1.30.1 | DNA + H2O |
resolving of DNA-four way junction |
3.1.30.1 | DNA + H2O |
resolving of four-way junction |
3.1.30.1 | DNA + H2O |
T7 endonuclase I resolves four-way junctions by simultaeously introducing two nicks on the two continously stacked strands at sites 5' to the junction, also acts on a variety of DNA structures |
3.1.30.1 | heteroduplex DNA + H2O |
cleavage of single base pair mismatches in heteroduplex DNA |
3.1.30.1 | more |
the enzyme is active on DNA and RNA, the enzyme endonucleolytically degrades ssDNA and RNA by 3'-5' mode to produce 5'-oligonucleotides and 5'-mononucleotides. It preferentially degrades poly(U), substrate specificity, overview |
3.1.30.1 | more |
ENDO3 is the only nuclease, of all members of the Arabidopsis S1-like nuclease family, that demonstrates activity characteristic of fungal S1-type nucleases and mung bean plant nuclease, i.e. it digests ssDNA under acidic pH and in the presence of Zn2+ |