EC Number   |
Natural Substrates |
|---|
   2.4.99.18 | Campylobacter jejuni heptasaccharide bound to undecaprenyl diphosphate + pilin |
alpha-1,3-linked sugar, in vivo in Escherichia coli |
| 2.4.99.18 | dolichyl diphosphooligosaccharide + protein L-asparagine |
- |
| 2.4.99.18 | dolichyl diphosphooligosaccharide + [protein]-L-asparagine |
- |
| 2.4.99.18 | dolichyl diphosphooligosaccharide + [protein]-L-asparagine |
asparagine-linked glycosylation is a common posttranslational modification of diverse secretory and membrane proteins in eukaryotes, where it is catalyzed by the multiprotein complex oligosaccharyltransferase. Eukaryotic oligosaccharyltransferase is a multifunctional enzyme that acts at the crossroads of protein modification and protein folding |
| 2.4.99.18 | dolichyl diphosphooligosaccharide + [protein]-L-asparagine |
oligosaccharyl transferase (OT) is a multisubunit enzyme that catalyzes N-linked glycosylation of nascent polypeptides in the lumen of the endoplasmic reticulum |
| 2.4.99.18 | dolichyl diphosphooligosaccharide + [protein]-L-asparagine |
oligosaccharyltransferase complex catalyzes the transfer of a lipid-linked core oligosaccharide onto asparagine residues of nascent polypeptide chains in the lumen of the endoplasmic reticulum |
| 2.4.99.18 | dolichyl diphosphooligosaccharide + [protein]-L-asparagine |
PglB, the key enzyme of the Campylobacter jejuni N-glycosylation system, transfers O polysaccharide from a lipid carrier (undecaprenyl pyrophosphate) to an accept or protein. PglB is the only protein of the bacterial N-glycosylation machinery both necessary and sufficient for the transfer |
| 2.4.99.18 | dolichyl diphosphooligosaccharide + [protein]-L-asparagine |
transfer of a preassembled, uniform oligosaccharide (Glc3Man9GlcNAc2 in most eukaryotes) from the isoprenoid lipid carrier dolichol diphosphate to the side-chain amide group nitrogen of an asparagine residue contained in a N-X-S(T) sequon of the polypeptide substrate, where X can be any amino acid except proline, mechanism, overview |
| 2.4.99.18 | dolichyl diphosphooligosaccharide + [protein]-L-asparagine |
transfer of a preassembled, uniform oligosaccharide (Glc3Man9GlcNAc2 in most eukaryotes) from the isoprenoid lipid carrier dolichol pyrophosphate to the side-chain amide group nitrogen of an asparagine residue contained in a N-X-S(T) sequon of the polypeptide substrate, where X can be any amino acid except proline, mechanism, modeling, overview |
| 2.4.99.18 | Escherichia coli group 1 K30 capsule antigen bound to undecaprenyl diphosphate + pilin |
in vivo in Escherichia coli |
