EC Number   |
Natural Substrates |
|---|
   2.1.1.228 | more |
Trm5 catalyzes methyl transfer to synthesize the m1G37 base at the 3' position adjacent to the tRNA anticodon |
| 2.1.1.228 | more |
TrmD catalyzes methyl transfer to synthesize the m1G37 base at the 3' position adjacent to the tRNA anticodon |
| 2.1.1.228 | more |
bifunctional Trm5a from Pyrococcus abyssi (PaTrm5a) catalyses not only the methylation of N1, but also the further methylation of C7 on 4 demethylwyosine at position 37 to produce isowyosine (EC 2.1.1.228 and EC 2.1.1.282, respectively) |
| 2.1.1.228 | more |
TrmD is a bacterial enzyme with a trefoil-knot in the active site, involving three crossings of the protein backbone through a loop. TrmD catalyzes methyl transfer from AdoMet to the N1 of G37 on the 3' side of the tRNA anticodon |
| 2.1.1.228 | more |
TrmD synthesizes the methylated m1G37 on bacterial tRNAs that contain both G37 and a preceding G36, the 3'-nucleotide of the anticodon |
| 2.1.1.228 | S-adenosyl-L-methionine + 4-demethylwyosine |
- |
| 2.1.1.228 | S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine |
- |
| 2.1.1.228 | S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe |
- |
| 2.1.1.228 | S-adenosyl-L-methionine + guanine37 in tRNA |
- |
| 2.1.1.228 | S-adenosyl-L-methionine + guanine37 in tRNA |
Trm5p is responsible for m1G37 methylation of mitochondrial and cytoplasmic tRNAs |
