EC Number   |
Natural Substrates |
|---|
   2.1.1.213 | 2 S-adenosyl-L-methionine + guanine10 in tRNA |
depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures |
| 2.1.1.213 | S-adenosyl-L-methionine + guanine10 in tRNA |
depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures |
