EC Number |
Natural Substrates |
---|
2.1.1.169 | more |
stepwise methylation of tricetin involving deprotonation of its hydroxyl groups by a His262-Asp263 pair followed by nucleophilic attack of SAM-methyl groups, substrate binding is mediated by an extensive network of H-bonds and van der Waals interactions, residue Val309 determines substrate specificity for tricetin and may define the evolutionary differences between the two closely related proteins, COMT and flavone OMT, molecular modeling, overview |
2.1.1.169 | S-adenosyl-L-methionine + 3',5'-O-dimethyltricetin |
- |
2.1.1.169 | S-adenosyl-L-methionine + 3'-O-methyltricetin |
- |
2.1.1.169 | S-adenosyl-L-methionine + tricetin |
- |
2.1.1.169 | S-adenosyl-L-methionine + tricetin |
i.e. 5,7,3',4',5'-pentahydroxyflavone, most preferred substrate |