EC Number   |
Natural Substrates |
|---|
   3.4.22.49 | cohesin + H2O |
cohesin Is cleaved with similar kinetics and timing near the centromere and telomere. Separase inhibition of PP2ACdc55 is necessary for robust cleavage of cohesin |
| 3.4.22.49 | cohesin + H2O |
the enzyme cleaves the kleisin subunit of cohesin and opens the cohesin ring to allow chromosome segregation |
| 3.4.22.49 | CPAR-1 protein + H2O |
separase cleaves the N-tail of the CENP-A related protein CPAR-1 at the meiosis I metaphase-anaphase transition |
| 3.4.22.49 | kendrin + H2O |
- |
| 3.4.22.49 | kendrin + H2O |
kendrin, also named pericentrin and specifically cleaved at a consensus site (R2231), is a crucial substrate for separase at the centrosome, protecting the engaged centrioles from premature disengagement and thereby blocking reduplication until the cell passes through mitosis |
| 3.4.22.49 | more |
auto-cleavage of separase coordinates multiple aspects of the G2/M programme in human cells, thus contributing to the timing and efficiency of chromosome segregation |
| 3.4.22.49 | more |
deletion of separase specifically blocks sister chromatid separation but not other aspects of mitosis, mitotic exit, cytokinesis, or even chromosome replication |
| 3.4.22.49 | more |
downregulation of PP2A(Cdc55) phosphatase by separase initiates mitotic exit. At anaphase onset, PP2A(Cdc55) activity is downregulated in a separase-dependent manner, triggering a first wave of Cdk-dependent Net1 phosphorylation and Cdc14 release. Separase is the essential trigger for Cdc14 activation in anaphase |
| 3.4.22.49 | more |
Hela cells lacking separase are delayed or arrested at the G2-M phase transition. Without separase, cells also have a prolonged prometaphase. Separase is required at multiple cell cycle stages including the interphase. Its role in promoting loss of sister chromatid cohesion might be important preferentially at arms but not centromers |
| 3.4.22.49 | more |
MAP kinase is required for the increased securin-separase interaction that rescues separase mutants under stresses |
