EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Reference |
---|
5.4.99.13 | -999 |
- |
size exclusion chromatography yields an estimated molecular mass of 135 kDa, consistent with the large subunit being a homodimer. The recombinant small subunit of PCM is purified as N-terminally His6-tagged protein and elutes with an apparent mass of 20 kDa in gel filtration, suggesting that it exists as a monomer based on the predicted mass of the polypeptide of 17.7 kDa. Gel filtration of a 1:1 mixture of the large and small subunits ofthe enzyme in presence of 5'-deoxyadenosylcobalamin, shows no evidence of complex formation, indicating weak interaction between the subunits under these conditions |
748193 |
5.4.99.13 | 14300 |
- |
alpha2beta2, IcmA2IcmB2, 2 * 62500 + 2 * 14300, gel filtration, IcmB provides the cobalamin-binding domain |
648802 |
5.4.99.13 | 20000 |
- |
recombinant detagged enzyme, small subunit monomer, gel filtration |
748193 |
5.4.99.13 | 62500 |
- |
alpha2beta2, IcmA2IcmB2, 2 * 62500 + 2 * 14300, gel filtration, IcmB provides the cobalamin-binding domain |
648802 |
5.4.99.13 | 65000 |
- |
IcmA, 1 * 65000 + several proteins of lower mass, SDS-PAGE |
648803 |
5.4.99.13 | 135000 |
- |
recombinant detagged enzyme, large subunit dimer, gel filtration |
748193 |
5.4.99.13 | 153000 |
- |
gel filtration |
648802 |