EC Number   |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Reference |
|---|
    4.1.1.90 | -999 |
- |
determination of disulfide bond formation in purified two-chain carboxylase and P80L and P378L two-chain carboxylases by SDS-PAGE and Western Blot analyses |
696271 |
| 4.1.1.90 | 33000 |
- |
cells transfected with the CAT-V5/His plasmid, Western Blot |
697705 |
| 4.1.1.90 | 60000 |
- |
proteinase K digestion of the hGC-FLAG reveals a 60 kDa fragment, indicating the lumenal location of the FLAG tag and therefore the carboxyl-terminus of the carboxylase. In contrast, FLAG-hGC does not show a proteinase Kresistant fragment except for the residual undigested full-length carboxylase, which indicates the cytoplasmic location of the FLAG tag and therefore the amino-terminus of the carboxylase |
696951 |
| 4.1.1.90 | 77000 |
- |
single major band on SDS gel electrophoresis. The eluted protein contains both stable vitamin K-dependent carboxylase and vitamin K epoxidase activity |
438307 |
| 4.1.1.90 | 85700 |
- |
calculated from sequence |
697786 |
| 4.1.1.90 | 87540 |
- |
calculated from sequence |
701214 |
| 4.1.1.90 | 94000 |
- |
SDS-PAGE |
696184, 698705, 701022, 701214 |
| 4.1.1.90 | 94000 |
- |
SDS-PAGE and Western Blot, His6-carboxylase-Ac-FLEEL confirming affinity-purified carboxylase |
698697 |
| 4.1.1.90 | 95000 |
- |
full-length carboxylase |
696951 |
| 4.1.1.90 | 99000 |
- |
SDS-PAGE, Western Blot. CHO cells transfected with the cDNA for wild type FLAG-vitamin K-dependent gamma-glutamyl carboxylase |
438309 |
