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EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Reference
3.4.24.74
-999
-
analysis of BFT-2 by native PAGE reveals bands at 36000 Da, 90000 Da and 200000 Da. Both the 36000 Da and higher-molecular-mass bands are biologically active when tested in the HT29/C1 cell assay
651483
3.4.24.74
18000
22000
gel fitration
31497
3.4.24.74
19000
-
1 * 19000, SDS-PAGE
31497
3.4.24.74
20000
-
gel electrophoresis and Western blot analysis, mature processed BFT in all mutants
668924
3.4.24.74
20000
-
Western blot analysis of cell-free culture supernatants of strain NCTC 9343 expressing mutants BFT-C2, BFT-C4 and BFT-A394E, and of whole cell lysates in preperations of strain NCTC 9343 expressing wild type BFT
668932
3.4.24.74
20000
-
x * 20000, SDS-PAGE
733433
3.4.24.74
20000
-
x * 44000, preenzyme, x * 20000, mature enzyme
683384
3.4.24.74
20600
-
1 * 20600, SDS-PAGE
31496
3.4.24.74
20600
-
cleaved to its enzymatically active secreted form
668836
3.4.24.74
21000
-
x * 21000, SDS-PAGE
651475
,
720923
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