EC Number   |
Metals/Ions |
Reference |
|---|
    3.5.4.2 | Co2+ |
11% of the activity with Mn2+ |
650622 |
| 3.5.4.2 | Fe2+ |
48% of the activity with Mn2+ |
650622 |
| 3.5.4.2 | Fe2+ |
purified enzyme contains 1.5 mol of Fe, 1.1 mol of Zn and 14 mol of Mn per mol. Enzyme uses either Fe(II) and/or Mn(II) as the catalytic metal |
755347 |
| 3.5.4.2 | Fe2+ |
reducing [FeIII/FeIII]-ADE with dithionite restores the deaminase activity |
718893 |
| 3.5.4.2 | Fe3+ |
[FeII/FeII]-ADE is oxidized to [FeIII/FeIII]-ADE with ferricyanide with inactivation of the deaminase activity |
718893 |
| 3.5.4.2 | Mn2+ |
purified enzyme contains 1.5 mol of Fe, 1.1 mol of Zn and 14 mol of Mn per mol. Enzyme uses either Fe(II) and/or Mn(II) as the catalytic metal |
755347 |
| 3.5.4.2 | Mn2+ |
required for activity |
172180 |
| 3.5.4.2 | Mn2+ |
required, optimal at 1-5 mM, inhibitory above |
650622 |
| 3.5.4.2 | more |
Mg2+, Mo2+, and Ca2+ have either no effect or only a weak effect on enzyme activity |
734047 |
| 3.5.4.2 | more |
the apo-enzyme is prepared and reconstituted with Fe2+, Zn2+, or Mn2+. In each case, two enzyme-equivalents of metal are necessary for reconstitution of the deaminase activity |
718893 |
