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EC Number Metals/Ions Commentary Reference
Display the reaction diagram Show all sequences 3.4.23.51Cd2+ conserved amino-acid residues involved in cadmium ligation in the crystal are essential for the endoproteolytic activity in HycI 651066
Display the reaction diagram Show all sequences 3.4.23.51Ni2+ HycI shows an open conformation at the putative nickel-binding site. Ni2+ has lower binding affinity with HycI than that of Cd2+, which is likely required for HycI to dissociate from HycE after the C-terminal processing 680866
Display the reaction diagram Show all sequences 3.4.23.51Ni2+ the reaction requires nickel to be bound to the precursor and the protease does not have a function in nickel delivery to the substrate. Radioactive labelling of cells with 63Ni, devoid of endopeptidase, resolves several forms of the precursor which are possibly intermediates in the maturation pathway. The endopeptidase uses the metal in the large subunit of [NiFe]-hydrogenases as a recognition motif 651066
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