EC Number   |
Metals/Ions |
Reference |
|---|
   3.4.21.66 | Ca2+ |
required |
732871 |
| 3.4.21.66 | Ca2+ |
required, two calcium sites (Ca1 and Ca2) are present in thermitase are likely to be present in proteolysin as well |
731179 |
| 3.4.21.66 | Ca2+ |
the enzyme binds very strongly one Ca2+, that cannot be removed without denaturation by any known method. A Ca2+ from a second binding site can be removed completely using Ca2+ chelating reagents |
29544 |
| 3.4.21.66 | Ca2+ |
three calcium binding sites, two of these are tight binding sites, and removal of calcium causes unfolding and autolysis to occur. The third calcium is more weakly bound, its removal reduces the activity of the enzyme by 10%, the reduction being reversible |
29501 |
| 3.4.21.66 | Co2+ |
activates to 119% at 1 mM |
732254 |
| 3.4.21.66 | more |
bivalent metal cations increase the activity of proteolysin |
731179 |
