EC Number   |
Metals/Ions |
Reference |
|---|
   3.4.21.5 | Ca2+ |
- |
683502, 683698 |
| 3.4.21.5 | Ca2+ |
activates |
681663 |
| 3.4.21.5 | Ca2+ |
required |
717140, 717496, 717789 |
| 3.4.21.5 | Ca2+ |
required for thrombin activation by factor Xa |
710224 |
| 3.4.21.5 | K+ |
activates, can partially substitute for Na+, binding to Asp189 |
652512 |
| 3.4.21.5 | K+ |
two ion binding sites per enzyme molecule, binding structure, molecular basis of monovalent cation selectivity, overview, the K+-bound enzyme form shows key differences compared with the Na+-bound structure resulting in different kinetics of activation, overview |
683253 |
| 3.4.21.5 | Li+ |
activates, can partially substitute for Na+, binding to Asp189 |
652512 |
| 3.4.21.5 | more |
mutants show reduced specificity for monovalent cations compared to the wild-type enzyme |
652512 |
| 3.4.21.5 | more |
not affected by NaCl up to 600 mM |
653981 |
| 3.4.21.5 | more |
the cation-free enzyme form assumes a conformation where the monovalent cation binding site is completely disordered, the S1 pocket is inaccessible to substrate and binding to exosite I is compromised by an unprecedented shift in the position of the autolysis loop |
683253 |
