EC Number   |
Metals/Ions |
Reference |
|---|
   3.2.2.27 | Fe2+ |
the UDG possesses a [4Fe-4S] cluster, distant from the active site, which interacts with loop structures and is unessential to the activity but necessary for stabilizing the loop structures |
693611 |
| 3.2.2.27 | Iron-sulfur cluster |
the [4Fe-4S]-binding cluster is important for the DNA binding and catalytic activity |
723284 |
| 3.2.2.27 | KCl |
activates optimal at 25 mM, recombinant UDG |
690585 |
| 3.2.2.27 | Mg2+ |
highest activity at 5 mM Mg2+ |
750389 |
| 3.2.2.27 | Mg2+ |
strongly stimulating at physiological concentrations |
692963 |
| 3.2.2.27 | Mg2+ |
strongly stimulating at physiological concentrations, 10fold for Ung2 at 10 mM. Mg2+ increases the preference of hUNG2 toward uracil in ssDNA nearly 40fold |
692963 |
| 3.2.2.27 | more |
divalent cations are not required for the activity |
722659 |
| 3.2.2.27 | more |
not affected by Mg2+ and Mn2+ |
750695 |
| 3.2.2.27 | NaCl |
activates, optimal at 25 mM, recombinant UDG |
690585 |
| 3.2.2.27 | NaCl |
activates, optimally at 20-25 mM |
691896 |
