EC Number |
Metals/Ions |
Reference |
---|
3.2.1.209 | Ca2+ |
activation |
646607 |
3.2.1.209 | Ca2+ |
inhibition by EGTA can be 90% restored after addition of 0.1 mM |
135972 |
3.2.1.209 | Ca2+ |
requirement, protects from thermal inactivation, Km value 0.5 mM |
646594 |
3.2.1.209 | Ca2+ |
the calcium ion binds to the carbonyl oxygen and the Ogamma of residue T525, and to four water molecules that are in turn hydrogen-bonded to one of the two carboxylate groups of residues E279, E435, E438 and E503 |
753413 |
3.2.1.209 | more |
presence of divalent cation required |
753757 |
3.2.1.209 | more |
the enzyme activity os not sensitive to the presence of Ca2+, Mg2+ and Mn2+ at concentrations up to 3 mM |
716100 |
3.2.1.209 | Zn2+ |
30% stimulation of the EGTA-inhibited enzyme at 0.1 mM |
135972 |