EC Number   |
Metals/Ions |
Reference |
|---|
   3.1.99.B1 | Ca2+ |
at pH 9.3, Ca2+substantially stabilizes both complexes, wild-type-substrate and D201I/D204S-substrate complexes, but calcium ions do not support FEN catalysis and inhibit the reactions supported by viable metal cofactors |
717841 |
| 3.1.99.B1 | K+ |
monovalent salt effect is broad, with equal activities observed in 50 and 100 mM KCl |
719690 |
| 3.1.99.B1 | KCl |
60 mM, the activity increases 1.3-fold, 100 mM KCl result in significant inhibition of activity |
719797 |
| 3.1.99.B1 | Mg2+ |
addition of 1 mM MgCl2 increases the activity 1.4-fold. 10 mM MgCl2 results in greater than 90% inhibition |
719797 |
| 3.1.99.B1 | Mg2+ |
addition of magnesium is required for enzyme activity. Mg2+ can not be replaced by Ca2+, Mn2+, or Zn2+. The Mg2+ concentration dependence is weak, with an optimum of 2.5 mM and at least 50% activity between 1 and 10 mM |
719690 |
| 3.1.99.B1 | Mg2+ |
endonucleolytic cleavage of flap substrate is only supported by Mg2+ and Mn2+, the cleavage site preferences for each enzyme is altered in the presence of Mn2+ |
719796 |
| 3.1.99.B1 | Mg2+ |
enzyme form StolL-FEN-1 exhibits activity in the concentration range 0.5-10 mM |
719114 |
| 3.1.99.B1 | Mg2+ |
required |
716689, 717807, 717830 |
| 3.1.99.B1 | Mg2+ |
the overall reaction catalyzed by wild-type T5FEN requires three Mg2+ ions, binding kinetics and mechanism, overview |
717841 |
| 3.1.99.B1 | Mg2+ |
two Sm3+ ions occupy Mg2+ ion sites seen in the FEN1 DNA-free structure |
717471 |
