EC Number   |
Metals/Ions |
Reference |
|---|
   2.7.7.49 | Co2+ |
enzyme exhibits a strong preference to incorporate Sp-TTP alphaS isomer over Rp-TTP alphaS isomer in the presence of Mg2+. This stereoselective preference is decreased when Mg2+ is replaced with Mn2+ and Co2+ |
663342 |
| 2.7.7.49 | K+ |
- |
643415 |
| 2.7.7.49 | K+ |
100 mM KCl, 2fold stimulation |
643415 |
| 2.7.7.49 | K+ |
5 mM, 50% stimulation of activity with poly(dA-dT) |
643415 |
| 2.7.7.49 | K+ |
5 mM, stimulates rate of DNA synthesis by 20% |
643418 |
| 2.7.7.49 | K+ |
maximal incorporation of dTTP into poly(rA) template primed with oligo(dT)12 with 50 mM KCl |
643447 |
| 2.7.7.49 | K+ |
monovalent cation is not required for activity, 20 mM KCl causes 15% stimulation |
643411 |
| 2.7.7.49 | K+ |
monovalent cations are not required, but afforded a severalfold stimulation, optimal concentration is 100 mM |
643420 |
| 2.7.7.49 | K+ |
optimal activity at 50 mM KCl with poly(rA)*oligo(dT) and 10-100 mM KCl for poly(rC)*oligo(dG) |
643429 |
| 2.7.7.49 | K+ |
optimal activity with the 1.6 kb in vitro transcript corresponding to the 3' end of the plasmid RNA (pVXN15/NsiI, CCA transcript) at 300 mM KCl, optimal activity with poly(rC)-oligo(dG)12-18 at 200 mM |
643430 |
