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EC Number	Metals/Ions	Commentary	Reference
2.5.1.32	Mg2+	1 mM, 40-50% stimulating effect	676639
2.5.1.32	Mg2+	Mn2+ or Mg2+ required	637880
2.5.1.32	Mg2+	required	2941
2.5.1.32	Mg2+	stimulatory in presence of Mn2+	637882
2.5.1.32	Mn2+	-	2941
2.5.1.32	Mn2+	absolute requirement for Mn2+, half-maximal activity at 0.2 mM. No other divalent cation can substitute efficiently for Mn2+	637872
2.5.1.32	Mn2+	absolute requirement, optimum at 1 mM	637882
2.5.1.32	Mn2+	maximal activity at 0.25 mM	637884
2.5.1.32	Mn2+	Mn2+ and ATP in combination are essential for catalytic activity, the effect is stoichiometric from 0.5-2.0 mM. Km for Mn2+ is 0.4 mM	637885
2.5.1.32	Mn2+	Mn2+ or Mg2+ required	637880

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Release 2023.1 (January 2023)