EC Number |
Metals/Ions |
Reference |
---|
2.5.1.1 | Co2+ |
required for the production of geranyl diphosphate |
738382 |
2.5.1.1 | Co2+ |
with dimethylallyl diphosphate and isopentenyl diphosphate the enzyme is far more active with Co2+ as an additive than with any other tested metal ion. In the presence of Co2+ or Mn2+, with dimethylallyl diphosphate as a cosubstrate, the enzyme produces about 96% geranyl diphosphate and only 4% farnesyl diphosphate. In contrast, with Mg2+ as an additive, PcIDS1 produces 18% geranyl diphosphate and 82% farnesyl diphosphate |
723664 |
2.5.1.1 | Mg2+ |
required |
739121 |
2.5.1.1 | Mg2+ |
required for activity |
738211 |
2.5.1.1 | Mg2+ |
required, activity reaches its highest value at 10 mM MgCl2. At 5 or 15 mM MgCl2, activity is about 65% of maximum |
706332 |
2.5.1.1 | Mg2+ |
with dimethylallyl diphosphate and isopentenyl diphosphate the enzyme is far more active with Co2+ as an additive than with any other tested metal ion. In the presence of Co2+ or Mn2+, with dimethylallyl diphosphate as a cosubstrate, the enzyme produces about 96% geranyl diphosphate and only 4% farnesyl diphosphate. In contrast, with Mg2+ as an additive, PcIDS1 produces 18% geranyl diphosphate and 82% farnesyl diphosphate |
723664 |
2.5.1.1 | more |
the enzyme is inactive without adding divalent metal cations |
723664 |