EC Number |
Metals/Ions |
Reference |
---|
2.3.3.3 | Ca2+ |
the enzyme contains stoichiometric amounts of Ca2+ (0.9 Ca/73 kDa) |
736357 |
2.3.3.3 | Co2+ |
can replace Mn2+ |
488158 |
2.3.3.3 | Co2+ |
Co2+ increases the activity by about 70% compared with that obtained withMn2+ |
736357 |
2.3.3.3 | Mg2+ |
Mg2+ has no effect at all on enzyme activity |
736357 |
2.3.3.3 | Mn2+ |
0.1 mM, required for maximal activity |
488157 |
2.3.3.3 | Mn2+ |
0.2 mM Mn2+ is required for full activity |
719729 |
2.3.3.3 | Mn2+ |
assays are performed in the presence of 0.2 mM MnCl2 |
736357 |
2.3.3.3 | more |
citrate synthase in extracts is not activated by Mn2+ |
488160 |
2.3.3.3 | Zn2+ |
activation below 0.02 mM, inhibition above |
488158 |
2.3.3.3 | Zn2+ |
the EDTA-inactivated enzyme regains activity by addition Zn2+ at final concentrations of 0.2 mM |
736357 |