EC Number |
Metals/Ions |
Reference |
---|
2.3.2.B11 | Ca2+ |
Asr1p is involved in calcium signalling. The interaction with calmodulin occurs in vivo and depends on physiological concentrations of Ca2+. A plataeu is reached at 100-500 nM CaCl2 |
724178 |
2.3.2.B11 | Ca2+ |
during purification on ubiquitin-Sepharose the Ca2+-dependent activity is lost and an essentially Ca2+-independent enzyme is obtained which is purified 90fold |
648929 |
2.3.2.B11 | Ca2+ |
Km: 0.005-0.010 mM |
648928 |
2.3.2.B11 | Ca2+ |
optimal concentration: 0.1 mM |
648928 |
2.3.2.B11 | Ca2+ |
required |
648927, 648928, 648929, 648930, 648931, 648932, 648933, 648934, 648935, 648936 |
2.3.2.B11 | Ca2+ |
the activity is regulated by the binding of the second messenger Ca2+ to the substrate calmodulin, which increases the activity ca. 10fold |
648937 |