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EC Number
Metals/Ions
Commentary
Reference
2.3.1.24
Ca2+
activates
752718
2.3.1.24
Mg2+
1.5fold activation at 2 mM
752695
2.3.1.24
Mg2+
activates
752435
,
752796
,
754086
,
754101
2.3.1.24
Mg2+
activates at 2 mM
752718
2.3.1.24
Mg2+
no absolute requirement for metal ions, magnesium ions stimulate the reaction
752793
2.3.1.24
Mn2+
activates
752718
2.3.1.24
more
poor effect observed by 0.001 mM of Cu2+, Zn2+, or Co2+
752718
2.3.1.24
more
the change of a Tris-Mg2+-DTT-NaF-NADPH-CHAPS buffer to a phosphate/K+-Mn2+-CHAPS buffer slightly improves the efficiency of Cer formation
720407
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