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EC Number Metals/Ions Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.18MgSO4 1 mM, addition to 5,5'-dithiobis(2-nitrobenzoic acid) assay mixture lacking EDTA inhibits, preincubation of transacetylase (2 mg/ml in 0.05 M Tris, pH 7.8) with MgSO4 for 1 h at 30°C and subsequent dilution of the enzyme 1000-fold in 5,5'-dithiobis(2-nitrobenzoic acid) assay mixture lacking both EDTA and the metal ion stimulates 486462
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.18Mn2+ stimulates when enzyme is initially incubated with the ion in Tris buffer but has no effect if initially incubated in phosphate or if added to the standard assay medium containing 0.05 M phosphate 486460
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.18more no requirement for divalent cation 486461
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