EC Number   |
Metals/Ions |
Reference |
|---|
    2.1.1.45 | KCl |
the optimal ionic strength for both enzymes requires 100 mM KCl, with DHFR displaying 2.5fold activation and TS 4.5fold activation |
743569 |
| 2.1.1.45 | Mg2+ |
- |
485357, 485372, 485380, 485431 |
| 2.1.1.45 | Mg2+ |
high requirement |
485431 |
| 2.1.1.45 | Mg2+ |
in Tris-acetate buffers 10 mM enhances enzyme activity by some 30% |
485377 |
| 2.1.1.45 | Mg2+ |
requirement depending upon the buffer |
485372 |
| 2.1.1.45 | Mg2+ |
TS is activated 4.5fold at 10 mM MgCl2, but inhibited at higher concentrations of MgCl2. The activating effect is not additive with activation by KCl |
743569 |
| 2.1.1.45 | more |
presence of Mg2+ does not enhance reaction rates and has no impact on the transition state structure of hydride transfer |
758166 |
| 2.1.1.45 | Na+ |
stimulates activity |
485402 |
