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EC Number	Metals/Ions	Commentary	Reference
6.3.2.32	Mg2+	the CofF protein strictly requires a divalent metal ion for ligase activity. CofF enzyme incubated with 10 mM MgCl2 has about 33% of the activity supported by 10 mM MnCl2	700925
6.3.2.32	Mn2+	the CofF protein strictly requires a divalent metal ion for ligase activity. CofF enzyme incubated with 10 mM MgCl2 has about 33% of the activity supported by 10 mM MnCl2	700925

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Release 2023.1 (January 2023)