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EC Number
Metals/Ions
Commentary
Reference
Ca2+
absolutely required, optimum concentration 1 mM
Ca2+
required for activity, all the mannuronan C5-epimerases of Azotobacter vinelandii show differences in concentration of calcium ions needed for full activity, overview; required for activity, all the mannuronan C5-epimerases of Azotobacter vinelandii show differences in concentration of calcium ions needed for full activity, overview; required for activity, all the mannuronan C5-epimerases of Azotobacter vinelandii show differences in concentration of calcium ions needed for full activity, overview; required for activity, all the mannuronan C5-epimerases of Azotobacter vinelandii show differences in concentration of calcium ions needed for full activity, overview; required for activity, all the mannuronan C5-epimerases of Azotobacter vinelandii show differences in concentration of calcium ions needed for full activity, overview; required for activity, all the mannuronan C5-epimerases of Azotobacter vinelandii show differences in concentration of calcium ions needed for full activity, overview; required for activity, all the mannuronan C5-epimerases of Azotobacter vinelandii show differences in concentration of calcium ions needed for full activity, overview
Ca2+
required, enhances gelation by 1.7fold following rSjC5-VI activity
Ca2+
required, optimum concentration 0.8 mM. The Ca2+ requirement of domain AlgE1-1 is much higher (optimum around 3 mM) than that of whole AlgE1, while domain AlgE1-2 needs slightly more Ca2+ than AlgE1 for optimal activity
Ca2+
required, optimum concentration 1-3 mM
Ca2+
the epimerization reaction is Ca2+ dependent, and both the A and R modules bind this cation. The R modules appear to reduce the Ca2+ concentration needed for full activity and also stimulate the reaction rate when positioned both N- and C-terminally
Mg2+
at Ca2+ concentrations lower than optimum, the addition of Sr2+ or Mg2+ has a stimulatory effect, but Mg2+ is not able to substitute for Ca2+ when no calcium is added
more
no Ca2+ ions required for activity, and the Ca2+-alginate complex is not a substrate for the enzyme
more
no effect by 1 mM of Mg2+, Sr2+, Li+, and Ba2+
NaCl
activates best at 300 mM
Results 1 - 10 of 12 > >>