EC Number   |
Metals/Ions |
Reference  |
|---|
    4.2.1.3 | Iron |
contains 2.1 mol of iron per mol of enzyme |
33794 |
| 4.2.1.3 | Iron |
crystallographic evidence for a three-iron center |
33798 |
| 4.2.1.3 | Iron |
inactive aconitase contains an oxidized [3Fe-4S]+cluster. Full activity is achieved with one electron per 3Fe cluster and at least 0.6 gatoms of Fe2+ per mol. The process involves building up of [4Fe-4S]2+ clusters |
33799 |
| 4.2.1.3 | Iron |
inactive aconitase contains a single [3Fe-4S]cluster |
33800 |
| 4.2.1.3 | Iron |
iron-sulfur enzyme |
33801 |
| 4.2.1.3 | Iron |
contains 2 gatoms of non-heme iron per mol of enzyme |
33795, 33803, 33805 |
| 4.2.1.3 | Iron |
cytosolic and mitochondrial isoenzyme require an intact [4Fe-4S] cluster. Mitochondrial aconitase is isolated predominantly in the [3Fe-4S] form (Fe/S ratio of 0.73) and must be activated by the addition of Fe2+. The cytoplasmic aconitase as isolated is about 80% active with a Fe/S ratio of 1.1 |
33811 |
| 4.2.1.3 | Iron |
the active form contains a [4Fe-4S]+ cluster, the inactive form contains a [3Fe-4S]+ cluster |
33812 |
| 4.2.1.3 | Iron |
Cys358, Cys421 and Cys424 are ligands to the Fe-S cluster in the inactive [3Fe-4S] form and the active [4Fe-4S] form |
33813 |
| 4.2.1.3 | Iron |
a significant proportion of the enzyme is in the inactive [3Fe-4S]1+ or apoenzyme forms. AcnB contains a much higher proportion of inactive enzyme than AcnA |
649675 |
