EC Number   |
Metals/Ions |
Reference |
|---|
   3.6.1.28 | Mg2+ |
required for catalytic activity, not required for substrate binding |
733393 |
| 3.6.1.28 | Mg2+ |
soluble enzyme requires Mg2+, enzyme from membrane is Mg2+-independent |
210019 |
| 3.6.1.28 | Mg2+ |
the addition of Mg2+ at 5 mM concentration enhances the rate of thiamine triphosphate hydrolysis by a factor of 17-20 |
758528 |
| 3.6.1.28 | Mg2+ |
the enzyme has an absolute requirement for divalent cations, binding of Mg2+ induces only a minor local conformational change |
687693 |
| 3.6.1.28 | Mg2+ |
the true substrate may be the Mg2+-thiamine-triphosphate complex |
210013 |
| 3.6.1.28 | Mn2+ |
0.1 mM |
667797 |
| 3.6.1.28 | Mn2+ |
30% of the activation with Mg2+ |
210020 |
| 3.6.1.28 | Mn2+ |
divalent cation requirement is satisfied by Mg2+, Mn2+ or Ca2+ |
210006, 210013, 210016 |
| 3.6.1.28 | Mn2+ |
maximal activation occurs at a cation to substrate ratio of 1:1 |
210006 |
| 3.6.1.28 | Ni2+ |
30% of the activation with Mg2+ |
210020 |
