EC Number |
Metals/Ions |
Reference |
---|
1.13.11.50 | Fe2+ |
utilizes a non-heme Fe2+ cofactor |
674150 |
1.13.11.50 | Fe2+ |
variable amounts |
636328 |
1.13.11.50 | Fe3+ |
citrate, no interference with Fe2+ |
696107 |
1.13.11.50 | Iron |
an interplay of residues Glu98, His104, Glu11 (from the neighbor subunit), and Arg80 is the most important for the Fe2+ transport in and out of the protein. The Fe2+ ion when expelled from the binding site can be trapped at different locations within the enzyme. The neighborhood of residue Glu11 (form the neighbor subunit) is the second most favorable binding site for the Fe2+ ion after the active site |
742322 |
1.13.11.50 | Iron |
dependent on, metalloenzyme, 1 iron bound per subunit, required for positioning of the substrate and for rendering of the appropriate electronic environment |
657866 |
1.13.11.50 | Mn2+ |
sulfate, similar binding as Fe2+ in wild-type enzyme |
696107 |
1.13.11.50 | more |
spectrophotometrical monitoring, Fe2+ and Fe3+ coordination, formation of iron(II) enzyme-substrate complexes, and detachment kinetics, overview |
725553 |
1.13.11.50 | more |
the enzyme is a mononuclear non-heme iron enzyme, overview |
711258 |
1.13.11.50 | Ni2+ |
dependent on |
764544 |
1.13.11.50 | Ni2+ |
sulfate, similar binding as Fe2+ in wild-type enzyme |
696107 |