EC Number |
Metals/Ions |
Reference |
---|
1.1.1.87 | Mg2+ |
Mn2+ or Mg2+ is required for this activity, and stronger activity is observed with Mn2+ |
760522 |
1.1.1.87 | Mg2+ |
required |
644540, 644541 |
1.1.1.87 | Mg2+ |
required, three conserved aspartate residues, D243, D267 and D271, coordinate Mg2+, which is also coordinated to the alpha-carboxylate and alpha-hydroxyl of homoisocitrate |
739958 |
1.1.1.87 | Mg2+ |
stimulation of oxidative decarboxylation, required for reductive carboxylation |
644539 |
1.1.1.87 | Mn2+ |
- |
660907 |
1.1.1.87 | Mn2+ |
Mn2+ or Mg2+ is required for this activity, and stronger activity is observed with Mn2+ |
760522 |
1.1.1.87 | Mn2+ |
required |
660918 |
1.1.1.87 | Mn2+ |
stimulation of oxidative decarboxylation |
644539, 644540 |
1.1.1.87 | more |
selectivity of the activator site for monovalent ions, K+ is the best activator, and NH4+ and Rb+ are also activators of the reaction, while Cs+, Li+, and Na+ are not, overview. Substitution of potassium acetate for KCl changes the kinetic mechanism of HIcDH from a steady state random to a fully ordered mechanism with the binding of Mg-HIc followed by K+ and NAD+ |
696220 |
1.1.1.87 | NH4+ |
activates |
696220 |