EC Number   |
Metals/Ions |
Reference |
|---|
   3.6.1.28 | NO3- |
stimulation by anions with the following decreasing order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme. The effect of NO3- is maximal at pH 7-8, it is negligible at pH 6.5 |
210006 |
| 3.6.1.28 | Br- |
stimulation by anions with the following order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme |
210006 |
| 3.6.1.28 | Ca2+ |
the addition of Ca2+ at 5 mM concentration enhances the rate of thiamine triphosphate hydrolysis by a factor of 17-20 |
758528 |
| 3.6.1.28 | Mg2+ |
the addition of Mg2+ at 5 mM concentration enhances the rate of thiamine triphosphate hydrolysis by a factor of 17-20 |
758528 |
| 3.6.1.28 | Mg2+ |
the enzyme has an absolute requirement for divalent cations, binding of Mg2+ induces only a minor local conformational change |
687693 |
| 3.6.1.28 | Mg2+ |
the true substrate may be the Mg2+-thiamine-triphosphate complex |
210013 |
