   2.7.3.9 | cytoplasm |
enzyme in cells from dense colonies grown on agar plates with LB medium or synthetic Neidhardt medium shows primarily bipolar or punctated distribution (concentrated in numerous discrete spots throughout the cell). In liquid culture, under carefully defined carbon-limiting growth conditions cellular levels of active enzyme remain constant for each carbon source, but distribution depends on C source, cell density, growth phase, and apparently on conditioned medium. Determination is diffuse (dispersed throughout the cell) during exponential growth on LB medium or ribose/Neidhardt medium. On ribose distribution is punctate in the stationary phase, reverting to diffuse when more ribose is added. In LB medium, both wild-type enzyme and a nonphosphorylatable mutant, H189Q, show a diffuse distribution during growth, but, shortly after the addition of isopropyl beta-D-thiogalactopyranoside distribution of wild-type enzyme becomes bipolar, trhat of H189Q-Y-EI does not |
GO:0005737 AmiGO QuickGO |
663221 |
