EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
6.3.1.8 | 0.0224 |
- |
glutathione |
mutant enzyme R598A |
673330 |
6.3.1.8 | 0.052 |
- |
ATP |
pH 7.2, 25°C, recombinant trypanothione synthase performing the glutathionylspermidine synthase reaction |
662446 |
6.3.1.8 | 0.059 |
- |
spermidine |
pH 7.3, 25°C, synthetase activity |
649733 |
6.3.1.8 | 0.076 |
- |
spermidine |
mutant enzyme S337A |
673330 |
6.3.1.8 | 0.076 |
- |
spermidine |
wild type enzyme |
673330 |
6.3.1.8 | 0.078 |
- |
spermidine |
pH 7.3, 25°C, synthetase activity of the C79A mutant |
649733 |
6.3.1.8 | 0.096 |
- |
MgADP- |
pH 7.3, 25°C, synthetase activity of the C79A mutant |
649733 |
6.3.1.8 | 0.114 |
- |
MgADP- |
pH 7.3, 25°C, synthetase activity |
649733 |
6.3.1.8 | 0.126 |
- |
spermidine |
mutant enzyme S335A |
673330 |
6.3.1.8 | 0.13 |
- |
gamma-L-Glu-L-Cys(S-methyl)-Gly |
- |
966 |