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EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Reference
6.2.1.45	0.000073	-	ubiquitin carrier protein E2	mutant enzyme D576N	674561
6.2.1.45	0.000089	-	ubiquitin carrier protein E2	mutant enzyme K528A	674561
6.2.1.45	0.000111	-	ubiquitin carrier protein E2	wild-type enzyme	674561
6.2.1.45	0.000122	-	ubiquitin carrier protein E2	mutant enzyme D576E	674561
6.2.1.45	0.000135	-	ubiquitin carrier protein E2	mutant enzyme D576A	674561
6.2.1.45	0.00017	-	Oregon Green-labeled ubiquitin	pH 7.5, 25°C	728208
6.2.1.45	0.00017	-	ubiquitin	pH 7.5, 25°C	728208
6.2.1.45	0.0008	-	ubiquitin	wild-type enzyme	674561
6.2.1.45	0.0011	-	ubiquitin	mutant D576E, pH 7.5, temperature not specified in the publication	674561
6.2.1.45	0.0011	-	ubiquitin	mutant enzyme D576E	674561

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Release 2023.1 (January 2023)