EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
6.1.1.24 | -999 |
- |
more |
detailed comparison of kinetic parameters between Methanothermobacter thermautotrophicus GluRSND and Escherichia coli recombinant hybrid GlnRS mutants, which are also capable of Glu-tRNAGln synthesis, overview |
714207 |
6.1.1.24 | -999 |
- |
more |
detailed comparison of kinetic parameters between recombinant hybrid GlnRS S1/L1/L2 and other recombinant hybrid mutant enzymes, with the naturally occurring Methanothermobacter thermautotrophicus GluRSND, which is also capable of Glu-tRNAGln synthesis, overview. Both kcat and Km for glutamate are recapitulated in the engineered enzyme, but Km for tRNA is 200fold higher |
714207 |
6.1.1.24 | -999 |
- |
more |
kinetics |
675400 |
6.1.1.24 | -999 |
- |
more |
kinetics and binding constants |
693653 |
6.1.1.24 | -999 |
- |
more |
kinetics of the mutant enzyme compared to wild-type GlnRS, EC 6.1.1.18, overview |
694903 |
6.1.1.24 | 0.000038 |
- |
tRNAGln |
recombinant hybrid GlnRS S1/L1/L2, pH not specified in the publication, temperature not specified in the publication |
714207 |
6.1.1.24 | 0.00045 |
- |
tRNAGln |
homologous tRNAGln, in absence of regulatory factor beta |
492293 |
6.1.1.24 | 0.00065 |
- |
tRNAGln |
homologous tRNAGln, in presence of regulatory factor beta |
492293 |
6.1.1.24 | 0.00079 |
- |
tRNAGlu |
- |
675400 |
6.1.1.24 | 0.00083 |
- |
tRNAGlu |
homologous tRNAGlu, in absence of regulatory factor beta |
492293 |